Expression evaluation of cell cycle regulatory genes (P27, P21, P14) in AML patient
Naser Shagerdi Esmaeli1, Atousa Homafar2, Mohyedin Barzegar1, Mohammad Rafiee1, Mohammad Hossein Mohammadi1, Mehdi Allahbakhshian Farsani1*
* Corresponding author
Department of Haematology and Blood banking Shahid Beheshti University of Medical Sciences, Tehran, Iran1
Department of nutrition science, Tabriz Branch, Islamic Azad University, Tabriz, Iran2
INTRODUCTION: Cell cycle control has a critical role in normal haematopoiesis, leukomogenesis can affect key cell cycle regulators such as CDI gene family.P27, P21 and P14 in CKI family are frequently involved in AML.Their defects disrupt the balance between proliferation and apoptosis which can be led to cancer formation.
OBJECTIVES: The present study aimed at expression investigation of such essential cell cycle proteins (P21, P27, P14) in bone marrow biopsies and peripheral blood obtained from newly diagnosed acute myeloid leukaemia patients.
METHODS: The results of present study obtained from bone marrow and peripheral blood sample of 93 newly diagnosed AML patients (39 males and 54 female classified in 3 age group ;< 35, 35-60 and >60 years old) obtained from Taleghani hospital. We have 45 APL patient and 48 Non M3 patient. In addition expression of target genes was detected compared with ABL as housekeeping gene using the ΔΔCT method.
RESULTS: P21 and P27 had 1.44 and 4.10 higher expression respectively in comparison with normal control group (PV>0.05), and average expression fold change of P14 compared with the control group was 3.69 that shown significant reduction (Pv˃0.05). We found no correlation between expression level of the mentioned genes and common AML prognostic factors including: age, gender, blast cell type and even sample type.
CONCLUSION: We hypothesized that at the first step they get rid from their anti-tumour function and at the second step the malignant cells employ these proteins at their service.
Key words: AML, Regulatory cell cycle proteins, P27, P21, P14, Expression evaluation